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. 2015 Sep 22;56(10):6049–6057. doi: 10.1167/iovs.15-17360

Figure 3.

Figure 3

(Upper panels) NFL from retinal explants cultured under hypoxia or hypoxia/reoxygenation show staining for calpain-specific breakdown product SBDP150kDa (red under white arrows) at 16 and 24 hours. Images were merged with Hoechst 33342-stained sections (blue). (Middle panels) Immunohistochemical staining of retinal explants show neuronal marker β-III tubulin (green) localized mostly in the NFL. (Lower panels) Double-stained retinal explants cultured under hypoxia/reoxygenation conditions show immunohistochemical colocalization of SBDP150kDa (red) and neuron-specific β-III tubulin (green) merged to form a yellow band of proteolyzed α-spectrin by calpains in the NFL. Abbreviations as in Figure 2 legend.