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(A, B) Normoxic controls. Flat mounts of retinal explants cultured under normoxia at 16 and 24 hours, show the NFL was stained for β-III tubulin (green), but not for the breakdown product SBDP150kDa (red). (C, D, middle panels) After hypoxia, abundant SBDP150kDa was found in the NFL. (E, F, lower panels) Formation of SBDP150kDa was almost completely inhibited by calpain inhibitor SNJ-1945.
