Table 1. Structural parameters obtained from the SAXS data of CTNNBL1 in various NaCl concentrations.
| NaCl (mM) | Rg,Ga (Å) | Rg,p(r)b (Å) | Dmaxc (Å) | I(0)Absd | MMe (kDa) | Conformation |
|---|---|---|---|---|---|---|
| Crystal | 34.89 ± 0.008 | 34.87 ± 0.018 | 106.0 | NDf | 110 | dimer |
| 0 | 41.80 ± 0.385 | 42.97 ± 0.140 | 135.0 | 0.1466 | 101 | dimer |
| 100 | 44.20 ± 0.541 | 45.73 ± 0.165 | 146.9 | 0.1834 | 126 | dimer |
| 200 | 46.40 ± 0.752 | 49.53 ± 0.221 | 163.5 | 0.1604 | 110 | dimer |
| mutantf | 42.11 ± 1.850 | 43.94 ± 2.310 | 163 | 0.0841 | 58 | monomer |
aRg,G (radius of gyration) was obtained from the scattering data by the Guinier analysis.
bRg,p(r) (radius of gyration) was obtained from the p(r) function by the program GNOM.
cDmax (maximum dimension) were obtained from the p(r) function by the program GNOM.
dI(0) (forward scattering intensity) was obtained from the scattering data by the Guinier analysis.
eMM (molecular mass) was estimated from absolute I(0) intensity.
fMutant stands for CTNNBL1L87R/I101R/G148R/Q192R/Q199R/Q238A/E278A/E282A.