Table 1. Summary of HDR experiments for generating cell lines with site-specific gene insertion.

Selection Method	DNA Size (kb)	Total Clone Numbers	Positive Clone Numbers	Positive Rates
Positive/Negative	4.2	132	31	23%
Positive	4.2	192	104	54%
No Dug	4.2	362	22	6%
Large Fragment	9.4	96	23	24%

The “large fragment” used the positive and negative selection method.