Figure 4. LINGO-1 antibody promotes parahippocampal cortex (PHC) remyelination in EAE mice.

(a,b) The EAE mice exhibited demyelination in the PHC and fimbria-fornix. After a six-week treatment with the LINGO-1 antibody, the level of MBP was significantly increased in the PHC, but no significant change was observed in the fimbria-fornix. (c) We found a severe reduction in the expression of kinesin light chain (KLC), in the PHC of the EAE mice compared with controls, and the KLC expression was restored in the anti-LINGO-1 treated mice. (e)No significant differences were found for dynein (DYN), in the PHC of the EAE mice and the LINGO-1 antibody treated mice. (g) A reduction in the expression of neurofilament 200 (NF200), was detected in the EAE mice, and a slight increase was observed in the LINGO-1 antibody-treated mice. (d,h) KLC/NF200 expression was also decreased in the fimbria-fornix of the EAE but was not restored to normal levels in the EAE mice treated with the LINGO-1 antibody. (f) There was no significant difference in the expression of DYN among the three groups of mice. (i–k) Immunohistochemical staining for MBP in the three groups (l) Medial entorhinal cortex (MEnt) is an important subregion of the parahippocampal cortex. Schematic diagram display the scope we get the image in the microscope. (j) One-way ANOVA with least significance difference (LSD) test was used to determine statistical significance of MBP. The mean OD value was decline in the EAE mice and after LINGO-1 treatment, it was increased. n(Ctrl) = 3, n(EAE) = 4, n(Anti-LINGO-1) = 3. *Denotes statistical significance compared with controls (P < 0.05). **Denotes statistical significance compared with controls (P < 0.01). ^#Denotes statistical significance compared with EAE mice without treatment (P < 0.05). ^##Denotes statistical significance compared with EAE mice without treatment (P < 0.01). Scale bar, 100 μm.