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. 2015 Sep 18;5:14263. doi: 10.1038/srep14263

Figure 6. Transcription of ompS38 initiates from two sites.

Figure 6

(A) Constructs of the S. oneidensis ompS38 promoter mutants. Constructs were named either as strains for OmpS38 production analysis shown in (B) or promoters for the lacZ reporter analysis shown in (C). For instance, ∆414-203 refers to a mutant lacking base-pairs from -414 to -203 relative to the ompS38 gene and P202 means the resulting segment (from -202 to -1) in ∆414-203 is used to drive the lacZ gene. P414 refers to the wild-type promoter PompS38 in Fig. 5, covering base-pairs from -414 to -1. (B) SDS-PAGE analysis of OM proteins in strains indicated. Gels shown here were run under the same experimental conditions. (C) Expression analysis of promoters indicated in WT and ∆crp strains. (D) Nitrite susceptibility assay of strains indicated. Cells were prepared as described in Fig. 3A. ∆cydX is hypersensitive to nitrite. Pnon, no promoter in front of cydX. Experiments were conducted independently at least three times and error bars represent S.D. or the representative was presented.