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. 2015 Sep 18;5:14263. doi: 10.1038/srep14263

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(A) SDS-PAGE analysis of OM proteins in strains indicated. The assay included the wild-type strain with iron at different levels, which were achieved by adding FeCl₃ or iron-chelator dipyridyl. The lanes shown here were from the same gel, which was given in Fig. S3B. (B) SDS-PAGE analysis of OM proteins in the ∆fur strain under conditions indicated. In both (A,B), relative signal intensity (RSI) of OmpS38 in the indicated samples was estimated using software Image J. The averaged value for the wild-type in each gel was set to 1 as the standard, to which values of other samples were normalized. The values are the mean ± SD (error bars) (n = 4). (C) Expression analysis of P_ompS38 in strains under conditions indicated. Che represents chelator dipyridyl. Experiments were conducted independently at least three times and error bars represent S.D. or the representative was presented.