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. 2015 Sep 18;5:14209. doi: 10.1038/srep14209

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(a) Image of a field of human embryonic stem cells (hESCs) seeded at 1,500 cells/cm² over 3 days. (b) Manual analysis of the cells forming colonies and (c) cells failing to form colonies. We observed that production of granddaughter cells within 24 hrs of plating was strongly correlated with successful colony formation.