Table 2. Mutations identified in the temperature-adapted mutant TM29.
| Referencea | Variationb | Changed | Gene | Protein | Amino acid |
|---|---|---|---|---|---|
| 208637 | SNP | A→G | llmg_0219 | Putative membrane protein | Thr→Ala |
| 230889 | SNP | G→A | llmg_0242 | Probable transcriptional regulatory protein | Gly→Arg |
| 403714 | SNP | C→T | Intergenic | ||
| 598835 | SNP | C→T | pabC | Putative aminodeoxychorismate lyase | Pro→Ser |
| 905914 | SNP | G→A | trmD | tRNA (guanine-N(1)-)-methyltransferase | Val→Ile |
| 927877 | SNP | T→A | llmg_0962 | Transcriptional regulator, araC family | Cys→Stop |
| 945240 | DIP | —→G | tnp904 | Transposase for insertion sequence IS904F | Frameshift |
| 1078271 | SNP | A→G | pfk | 6-phosphofructokinase | Silent mutation |
| 1164619 | SNP | C→G | Intergenic | ||
| 1966122 | SNP | C→T | rpoC | DNA-directed RNA polymerase subunit beta′ | Gly→Arg |
| 1966323 | SNP | C→T | rpoC | DNA-directed RNA polymerase subunit beta′ | Glu→Lys |
| 2358022 | SNP | G→A | cdsA | Phosphatidate cytidylyltransferase | Pro→Leu |
| 2434453 | SNP | G→A | llmg_2477 | Lysine specific permease | Ala→Ser |
| 2497054 | SNP | G→A | llmg_2541 | Cation transporting ATPase | Ala→Thr |
| 1322282- | llmg_1349- | ||||
| DEL | — | — | — | ||
| 1334514 | llmg_1358 |
aReference position refers to the genome sequence of L. lactis MG1363 (GenBank accession # AM406671).
bSNP, single nucleotide polymorphism; DIP, deletion/insertion polymorphism; DEL, large deletion.