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. 2015 Sep 21;5:14274. doi: 10.1038/srep14274

Figure 3. Pre-treatment with NP-CpG recruits DCs and decreases Th2 cytokines in lungs of allergic mice.

Figure 3

Mice were pre-treated with CpG and then sensitized and challenged with HDM as in Fig. 2A. Analysis of dendritic cells (DCs) and CD4+ T cells in the lungs (A–C) and lung-draining lymph nodes (LNs) (D-F): (A,D) frequencies of all CD11c+ MHCII+ DCs as percentage of live cells, (B,E) relative expression levels (mean fluorescence intensity, MFI) of CD86 expression by CD11c+ MHCII+ DCs, and (C,F) frequencies of CD4+ T cells (as percentage of live cells). (G) Concentrations of indicated cytokines in lung homogenates as determined by ELISA. Data represent mean ± SEM from two independent experiments, 10 mice per group. *P < 0.05, **P < 0.01.