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. 2015 Sep 21;5:14274. doi: 10.1038/srep14274

Figure 5. Therapeutic delivery of NP-CpG reduces IgE, eosinophilia, and Th2 immunity in lungs of allergic mice.

Figure 5

Mice were first sensitized with 15 μg HDM on days 1, 3, and 5, then treated with 2 μg CpG (free or NP-conjugated) on days 15, 17, and 19, then challenged with 15 μg HDM on days 22, 24, and 26, and finally sacrificed on d 30. All solutions were delivered via the pulmonary route. (A) Experimental timeline. (B) Total IgE concentrations in serum at day 30 as determined by ELISA. (C) Numbers and (D) frequencies of macrophages, eosinophils, lymphocytes and neutrophils in the bronchoalveolar lavage (BAL) as defined by Diff Quick staining of cytospins. (E) Concentrations of indicated cytokines in lung homogenates as determined by ELISA. Data show mean ± SEM, 5 mice per group. *P < 0.05, **P < 0.01; #P < 0.05 compared to naïve mice.