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. 2015 Sep 22;5:14334. doi: 10.1038/srep14334

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(a) Absorption (black) and fluorescence emission (grey) spectra of FAD in phosphate buffered saline at pH = 7.0. (b,c) Fluorescence intensity image and the corresponding FLIM image of an unstained duct (optical section plane is indicated in the lower left) displaying the autofluorescence after excitation at λ_ex = 470 nm, with the following structural features: 1 cell nucleus, 2 luminal cuticle, 3 lumen, 4 trachea, 5 point-shaped structures. Recording parameters: 200 pixel × 200 pixel, 80 μm × 80 μm, pixel dwell time 0.4 ms/pixel, TCSPC time resolution 25 ps, repetition rate 20 MHz.