Skip to main content
. 2015 Sep 22;5:14076. doi: 10.1038/srep14076

Figure 1. Statin induction of the PEPCK1 gene mediated by SGK2 and PXR.

Figure 1

(a) Relative expression of PEPCK1 (left) and CYP3A4 (middle) mRNA levels measured by qRT-PCR in SGK2 siRNA-transfected human primary hepatocytes treated with simvastatin (Simva, 10 μM) for 24 h. Results are shown as fold change relative to DMSO treated cells. (n = 3, mean ± s.d) *P < 0.05, determined by, Student’s t test. Right, Western blot analysis of SGK2 and β-actin from whole cell lysates of each of these human primary hepatocytes. (b,c) Top, relative expression of PEPCK1 mRNA levels measured by qRT-PCR in SGK2 siRNA or PXR siRNA-transfected ShP51 cells treated with simvastatin (Simva, 10 μM) for 2.5 and 5 h. Results are shown as fold change relative to DMSO treated cells (n = 3, mean ± s.d) *P < 0.05, **P < 0.01, determined by Student’s t test. Bottom, Western blot analysis of SGK2, PXR and β-actin from whole cell lysates of each of these ShP51cells.