Table 1. Drugs used in the iterative search using the TACS algorithm.
Drug | Suggested Activity | Obtained from | Stock Conc. | Final Conc. |
---|---|---|---|---|
Sunitinib | Tyrosine kinase inhibitor39 | Lc Laboratories USA | 126.5 μM | 3.16 μM |
Rapamycin | mTOR (mammalian target of rapamycin) inhibitor40 | Sigma-Aldrich Sweden AB | 86.4 μM | 2.16 μM |
Trichostatin A | Histone deacetylase inhibitor41 | Sigma-Aldrich Sweden AB | 3.2 μM | 0.08 μM |
17-AAG | Heat shock protein (HSP) inhibitor42 | Lc Laboratories USA | 40 μM | 1 μM |
Mitomycin | Antitumour antibiotics43 | Sigma-Aldrich Sweden AB | 14.9 μM | 0.37 μM |
Afungin | Antifungal substance | Sigma-Aldrich Sweden AB | 0.4 μM | 0.01 μM |
Stock solution for all drugs were prepared 40 times higher than the final concentrations that were restored when diluted with cell suspension in the wells of the experimental plate.