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. 2015 Sep 22;5:14170. doi: 10.1038/srep14170

Figure 1. Polymerase activities of PB2 mutants in human cells cultured at different temperatures.

Figure 1

293T cells were transfected with a pFluc plasmid that expresses negative sense virus-like RNA encoding a firefly luciferase (Fluc) and a pRluc plasmid carrying the Renilla luciferase gene (Rluc) as an internal control (Promega). The 293T cells were also co-transfected with plasmids expressing the wild type (627K-701D, KD) and mutated PB2 (627E-701D, ED; 627K-701N, KN; or 627E-701N, EN), PB1, NP, and PA segments derived from the A/Anhui/1/2013(H7N9) virus. After culturing at 33 °C (a), 35 °C (b), 37 °C (c) and 39 °C (d) for 24 hours, cell lysate was used to measure Fluc and Rluc activity. Mean ± SD of triplicate experiments is shown. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.