Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Sep 22;5:14170. doi: 10.1038/srep14170

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2015, Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

PMC Copyright notice

Confluent monolayers of the cell lines were inoculated with recombinant viruses carrying mutations in the PB2 gene of the A/Anhui/1/2013 virus. Culture supernatants were harvested from human type II alveolar epithelial cells (A549) at 0, 12, 24, 36, 48, 60, 72, 84 and 96 hours post-inoculation (hpi) at 33 °C (a) and 37 °C (b), respectively. Virus titers were determined by TCID₅₀ assays using MDCK cells. Results are presented as mean ± SD of three repeated experiments. rgAH1-KD (wild type virus carrying PB2-627K and 701D), rgAH1-KN (with PB2-627K and 701N), and rgAH1-EN (with PB2-627E and 701N). *p < 0.05.