Table 2. Seroconversions of the C57BL/6 mice inoculated with recombinant H7N9 viruses.
| Virus | Inoculation dose (log TCID50/50μl) | HI titer |
MID50 (log TCID50) | ||||
|---|---|---|---|---|---|---|---|
| #1 | #2 | #3 | #4 | #5 | |||
| rgAH1-KD | 6 | ND | ND | ND | ND | ND | 1.1 |
| 5 | 160 | ND | ND | ND | 160 | ||
| 4 | 320 | 320 | 160 | 160 | ND | ||
| 3 | 160 | 160 | 160 | 160 | 160 | ||
| 2 | 160 | 160 | 80 | 80 | <10 | ||
| 1 | 160 | 160 | 160 | <10 | <10 | ||
| rgAH1-KN | 6 | ND | ND | ND | ND | ND | 0.9 |
| 5 | 160 | ND | ND | ND | ND | ||
| 4 | ND | ND | ND | ND | ND | ||
| 3 | 160 | 160 | ND | ND | 320 | ||
| 2 | 320 | 160 | 160 | 160 | 20 | ||
| 1 | 160 | 160 | 160 | 160 | 10 | ||
| rgAH1-EN | 6 | 320 | 160 | 160 | 40 | ND | 2.5 |
| 5 | 640 | 160 | 160 | 80 | 80 | ||
| 4 | 320 | 160 | 160 | 160 | 160 | ||
| 3 | 160 | 160 | 80 | 40 | 10 | ||
| 2 | 160 | <10 | <10 | <10 | <10 | ||
| 1 | <10 | <10 | <10 | <10 | <10 | ||
| PBS | <10 | <10 | <10 | <10 | <10 | ||
Serum collected at 14 days post-inoculation from each mouse (#1 to #5, respectively) was tested for heamagglutination inhibition (HI) titers against the A/Anhui/1/2013 (H7N9) virus. Median infectious dose (MID50) was determined using the Karber Method23. ND: Not determined due to death of the animal (treated as positive for determination of the MID50). HI titer <40 was regarded as negative for seroconversion.