Figure 4. Inhibitory effects on migration, invasion and proliferation.

(A) Migration inhibition of B16F10-Nex2 cells incubated with 0.3 nmole/10³ cells of C36L1 and SC36 for 24 h (B) Migration field examined by phase contrast microscopy at 0 and 24 h (magnification, ×100); (C) C36L1 inhibits invasion through Matrigel of B16F10-Nex2 cells, added 0.3 nmoles/10³ cells during 24 h; (D) Time dependent inhibition of B16F10-Nex2 cells proliferation by C36L1 at 0.5 nmoles/10³ cells; (E) Cell cycle of B16F10-Nex2 cells after incubation with C36L1 and SC36L1 at 0.5 nmoles/10³ cells for 18 hours. Percent tumor cells at G1, S and G2/M phases are indicated. (*p < 0.05; **p < 0.01; **p < 0.001, in relation to untreated control).