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. 2015 Sep 23;5:14307. doi: 10.1038/srep14307

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(A) SDS-PAGE analysis of expressed GDO in BL21 (DE3) on a 12.5% gel. Lane M, protein molecular weight marker (MBI); Lane 1, uninduced cells; Lane 2, cell culture induced with 1 mM IPTG; Lane 3, the supernatant of the induced cells; Lane 4, the precipitate of the induced cells; Lane 5, purified recombinant His₆-GDO protein; (B) NATIVE-PAGE of purified GDO-His₆. Lane M, protein molecular weight marker (MBI); Lane 1, 5 μl purified GDO-His₆.