Figure 4. Enzyme kinetics of the purified GDO.

A mixture containing 0.46 mM gentisate in 0.1 M phosphate buffer, pH 7.4, was mixed with an equal volume of (2.4 mg/ml) GDO. Absorbance was measured at times ranging from 0 s to 10 s at 4 °C at the UV absorbance spectra, 330 nm.