Figure 5. Development of in vitro 3D oxidative astrocytes model and confirmation of the positive effect of C-Pc on astrocytes.

MTT assay was implemented for (a) N-astrocytes and (b) H-astrocytes. mRNA expression of antioxidative enzymes such as MnSOD, CuZnSOD, ECSOD and catalase on (c) N-astrocytes cultured on 2D PCL film and 3D PCL nanofiber and (d) N-astrocytes, H-astrocytes and C·H-astrocytes cultured on 3D PCL nanofiber was measured by RT-qPCR. Data are presented as the means ± SEMs. The asterisk denotes significant difference against (a,b) day 1 and (d) N-astrocytes samples at each concentration. *p < 0.05 and **p < 0.01