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. 2015 Sep 24;5:13730. doi: 10.1038/srep13730

Figure 2. Michaelis-Menten kinetic analyses of phenothiazines on RCasp6.

Figure 2

Initial enzyme velocity of Casp6 plotted against indicated concentrations of Ac-VEID-AFC substrate in presence of 0, 10 μM, and 25 μM methylene blue (a), 0, 0.2 μM, and 0.4 μM azure A (b), or 0, 1 μM, and 5 μM azure B (c). The inset represents the Lineweaver-Burk plot for methylene blue (a), azure A (b), and azure B (c) with Ac-VEID-AFC substrate showing competitive mode of inhibition. Data represent the mean ± S.D. of three independent experiments.