Figure 5. The relationship between CD24/CD44 expression and transportability in SUM149 cells is investigated.

(a) Immunofluorescence staining is shown for CD24 (green), CD44 (red), and the merged image. The cells labeled 1 and 7 are CD24^High/CD44^Low phenotypes. The cells labeled 2, 5, and 6 are CD24^Low/CD44^High phenotypes. The cell labeled 4 is CD24^High/CD44^High phenotype. The cell labeled 3 is CD24^Low/CD44^Low phenotype, scale bar = 50 μm. (b) Microscopic images show CD24 and CD44 staining of trapped cells of indicated diameters and transportability, scale bar = 100 μm. (c) The proportion of CD24^High cells is plotted versus gap width. The proportion of cells expressing CD24 decreases with transportability. (d) The proportion of CD44^High cells is plotted versus gap width. The proportion of cells expressing CD44 increases with transportability. (e) The proportion of CD24^Low/CD44^High cells is plotted versus gap width. The proportion of cells with phenotype CD24^Low/CD44^High increases with transportability. (f) Transportability versus cell diameter is plotted for CD24^Low/CD44^High cells and SUM 149. Blue and black circles indicate the 80% confidence interval centered at the mean depicted by a green dot.