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. 2015 Sep 25;5:14389. doi: 10.1038/srep14389

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(A) Schematics of TDAP. See the main text for details of the method. (B) Spot assay of the indicated strains on YES plates, with or without hydroxyurea (HU). (C) BH-SUMO expressed from endogenous locus in strains with (+) or without (−) pAdh-driven BirA expression. SUMO conjugates were sequentially purified on Ni-NTA and streptavidin beads. Input and eluate after a single step (Ni) or tandem (Ni-Strep) purification were analyzed by Western blotting with a SUMO antibody. The asterisk marks the position of free BH-SUMO. Sypro Ruby staining of the input is shown on the left as a loading control.