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. 2015 Sep 25;5:14357. doi: 10.1038/srep14357

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(a) TD analysis with TaqI-digested DNA are lane 1, Nipponbare; lane 2, BDT1/BDT1; lane 3, BDT1/Bdt1; lane 4, Bdt1/Bdt1. 1 The arrowheads represent polymorphic bands (positions 1–4) among WT and Bdt1 plants. (b) Genotyping of progeny plants obtained from Bdt1/Bdt1 by PCR analysis. The filled and open arrowheads indicate PCR-amplified bands using primer sets that detect nDart1–3 insertion at positions I–IV. Insertion of nDart1 was detected in all of the progeny only at position II among four candidates.