. 2015 Sep 28;5:14437. doi: 10.1038/srep14437

Table 1. Rescue activities of CRN7 and mutant proteins in corB ⁻ on actin rod formation, phagocytosis and adhesion to a plastic surface.

graphic file with name srep14437-t1.jpg

Actin rod formation upon DMSO treatment. Cells were treated with DMSO (5%) and fixed with methanol after 10 minutes of incubation. Staining was with mAb act1 for actin and DAPI for nuclei. Between 100 and 200 cells were analyzed per experiment. The differences were statistically significant (P < 0.05).

Phagocytosis of corB⁻ and corB⁻ cells expressing the indicated CRN7 proteins. Cells were incubated with yeast cells and fixed at the indicated time points. Staining was with mAb act1 to visualize the cells. The data represent the mean from three indivdual experiments with 180, 180 and 400 cells evaluated each. Mean and SD are shown. The difference between AX2 and corB⁻ and corB⁻ GFP-MUT3 was statistically significant (P < 0.05). Care was taken to analyze cells expressing comparable levels of the GFP-fusion proteins.

Adhesion of CRN7 mutants to a plastic surface. The number of detached cells was determined after one hour of shaking at 100 rpm and set into relation to the total number of cells. The data are from 3 independent experiments in which each assay was performed in triplicate.

^*Detached cells.

Table 1. Rescue activities of CRN7 and mutant proteins in corB − on actin rod formation, phagocytosis and adhesion to a plastic surface.

Table 1. Rescue activities of CRN7 and mutant proteins in corB ⁻ on actin rod formation, phagocytosis and adhesion to a plastic surface.