Table 2. Analysis of random motility.
| AX2 | corB− | corB− GFP-CRN7WT | corB− MUT1 | |
|---|---|---|---|---|
| Speed (μm/min) | 4.46 ± 2.52 | 2.87 ± 1.28 | 5.41 ± 1.24 | 4.91 ± 1.06 |
| Direction change (deg) | 67 ± 13.45 | 67.81 ± 10.73 | 43.39 ± 13.36 | 41.01 ± 19.76 |
| Persistence (μm/min-deg) | 0.73 ± 0.46 | 0.56 ± 0.31 | 1.3 ± 0.49 | 1.39 ± 0.78 |
| corB− MUT2 | corB− MUT3 | AX2 WASP | corB− WASP | |
| Speed (μm/min) | 5.49 ± 2.67 | 3.51 ± 0.76 | 3.03 ± 1.26 | 3.22 ± 1.0 |
| Direction change (deg) | 55.45 ± 10.08 | 59.11 ± 7.15 | 69.67 ± 14.97 | 58.93 ± 13.25 |
| Persistence (μm/min-deg) | 1.21 ± 0.88 | 0.68 ± 0.2 | 0.55 ± 0.34 | 0.74 ± 0.33 |
Images were taken at 10 × magnification every 30 s. In all cases cells were recorded over a period of 30 min. Cells were recorded and after tracing of the cells the centroid of the cells was determined by computer-assisted analysis (DIAS). This allows calculations of speed, direction change and persistence. Direction change represents the average change of direction (in degrees) between two subsequent images. Persistence is an estimation of movement in the direction of the path. Values are mean ± standard deviation.