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. 2015 Sep 28;5:14569. doi: 10.1038/srep14569

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Culture media (CM) have been precipitated with ammonium sulphate (AmSO₄) and further purified by two sequential steps of RP-HPLC chromatography on C18 and C4 columns (bacteriocin peak is marked by an arrow). The composition of the fractions representing each step of purification is illustrated by SDS-PAGE.