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. 2015 Sep 28;5:14569. doi: 10.1038/srep14569

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(A) Sequence of the bacSp222 structural gene. The start and stop codons of the coding sequence are bolded. Upstream, a putative promoter −35 and −10 regions and a putative ribosome binding site (RBS) are underlined. Downstream, the reverse horizontal arrows indicate a putative transcription terminator. (B) Genetic organization of the bacSp222 gene cluster. Nucleotide numeration with respect to the first nucleotide in bacSp222 (denoted as 1 on the scale above the scheme). An inverted repeat downstream bacSp222 is marked with a hairpin. ORFs are marked with arrows in the scale. (C) Alignment of the amino acid sequence of BacSp222 and similar bacteriocins: lacticins Q and Z, aureocin A53, as well as epidermicin NI01 (prepared using CLC Workbench software).