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. 2015 Sep 28;5:14492. doi: 10.1038/srep14492

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(A) Levels of expression of Pl-TLR gene analysed by comparative qPCR with total RNA isolated from control (injected only with ASW) and exposed immune cells. Levels are expressed in arbitrary units as fold increase compared to controls assumed as 1, using the endogenous gene Pl-Z12-1 for normalization. Each bar represents the mean of three independent experiments ±SE. (B) Representative images of the TLR4-like protein levels of 3 control specimens and 3 specimens exposed to TiO₂NPs evaluated by immunoblotting. Histograms represent the means of the three independent experiments (18 specimens obtained from 3 independent experiments) ±SE after normalization with actin levels.