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. 2015 Sep 28;5:14470. doi: 10.1038/srep14470

Figure 2. ATF3 interacts with the p65 subunit of nuclear factor κB (NF-κB).

Figure 2

(a) Cells were treated with 1 μg/mL LPS for the indicated times. Beads were labelled with ATF3 antibody, and the interacting proteins, which were bound to ATF3, were precipitated. A total of 20 μg protein was separated with electrophoresis. The levels of these proteins were determined with Western blot using whole-cell lysates. (b) Cells were treated with 1 μg/mL LPS for the indicated times. Beads were labelled with p65 antibody, and the proteins bound to p65 were precipitated. A total of 20 μg protein was separated with electrophoresis. Protein levels were determined with Western blot using whole-cell lysates. Data show a representative set among the results of three independent experiments (n = 3).