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. 2015 Sep 28;5:14470. doi: 10.1038/srep14470

Figure 4. Inflammatory response genes in NF-κB signalling were up-regulated under ATF3-deficient conditions.

Figure 4

(a) Cells were transfected with 100 nM ATF3 siRNA for 48 h and then treated with 1 μg/mL LPS for 24 h. The secretion of interleukin (IL)-6 and nitric oxide (NO) were detected with ELISA. (b) RAW 264.7 cells were transfected with scrambled siRNA or ATF3 siRNA for 48 h and then treated with 1 μg/mL LPS for 2 h. Messenger RNA expression was measured using conventional reverse transcription-polymerase chain reaction. (c) RAW 264.7 cells were transfected with scrambled siRNA or ATF3 siRNA for 48 h and then treated with 1 μg/mL LPS for 2 and 4 h. Messenger RNA expression of IL-6 was measured using real-time polymerase chain reaction. Expression of IL-6 was normalized to β-actin and compared to control cells. Data represent three independent experiments (n = 3; *P < 0.05, **P < 0.01).