. 2015 Sep 25;6:8167. doi: 10.1038/ncomms9167

Table 3. Pre-incubation neutralization assay using 3BC176 and 3BC315.

JR-FL
Pre-incubation (h)	IC₅₀ (μg ml⁻¹)	Fold change	IC₅₀ (μg ml⁻¹)	Fold change	IC₅₀ (μg ml⁻¹)	Fold change
	3BC176		3BC315		35O22
1	0.30		5.84		0.0023
3	0.16	1.8	3.69	1.6	0.0028	0.8
6	0.11	2.7	2.34	2.5	0.0012	2.0
22	0.03	8.7	0.83	7.0	0.0015	1.5
	sCD4		PGT145		2G12
1	0.61		0.0064		0.12
3	0.33	1.8	0.0048	1.3	0.11	1.1
6	0.28	2.2	0.0041	1.5	0.13	1.0
22	0.13	4.9	0.0019	3.3	0.09	1.4
	D5		T-20
1	1,045		0.019
3	722	1.4	0.022	0.9
6	1,290	0.8	0.017	1.1
22	686	1.5	0.016	1.2

BG505
	3BC176		3BC315		35O22
1	1.93		1.62		0.0022
3	1.04	1.9	1.01	1.6	0.0011	2.1
6	0.82	2.4	0.71	2.3	0.0014	1.6
22	0.33	5.8	0.41	4.0	0.0011	2.0
	sCD4		PGT145		2G12
1	3.05		0.0096		0.092
3	2.74	1.1	0.0085	1.1	0.099	0.9
6	2.42	1.3	0.0099	1.0	0.101	0.9
22	1.19	2.6	0.0069	1.4	0.064	1.4
	D5		T-20
1	125		1.17
3	110	1.1	1.03	1.1
6	161	0.8	0.95	1.2
22	130	1.0	1.14	1.0

JR-FL or BG505 virus was incubated with antibody for various times, before adding to TZM-bl cells to test for infectivity. IC₅₀ values of 3BC315 and 3BC176 decrease as the antibody is pre-incubated with the viruses for longer times, indicating that these antibodies neutralize by inactivating Env over time. Fold change (decrease) in IC₅₀ is calculated relative to the standard 1h incubation time. Incubations resulting in a >3-fold change are shown in boldface.