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. Author manuscript; available in PMC: 2015 Nov 28.
Published in final edited form as: J Control Release. 2014 Aug 23;194:45–52. doi: 10.1016/j.jconrel.2014.08.010

Fig 5. Effect of gastric acid neutralization on stimulation of ovalbumin-specific serum IgGs.

Fig 5

Groups of mice (n=5 per group) were fed sodium bicarbonate buffer before orally administering ovalbumin (OVA)-based vaccine formulations. Vaccinations were performed on days 0 and 28, and serum samples were analyzed to determine anti-OVA IgG. (A) Titration curve of day 56 pooled mouse serum demonstrating the high anti-OVA IgG stimulation from groups receiving OVA with 5 mg LSs either with (LS5-Na group) or without (LS5 group) sodium bicarbonate pre-feed. No effect of gastric acid neutralization is seen. (B) Anti-OVA IgG in individual mouse serum at days 0 and 56 at a dilution of 1:400. Both LS5 and LS5-Na groups show significantly higher anti-OVA IgG response compared to OVA alone administered either with (OVA-Na group) or without (OVA group) sodium bicarbonate pre-feed. No significant difference is observed between LS5 and LS5-Na groups (p>0.05). Each symbol represents a mouse, the error bar represents standard deviation, and the horizontal line represents the mean. ns: not statistically significant. (C) Anti-OVA IgG subtypes in day 56 pooled serum samples (diluted 1:400). Error bars indicate standard deviation.