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. Author manuscript; available in PMC: 2016 Jun 23.
Published in final edited form as: Cell Rep. 2015 Jun 11;11(11):1714–1726. doi: 10.1016/j.celrep.2015.05.032

Figure 7. MDA5 function of IAV detection is conserved between mouse and human cells.

Figure 7

(A) Multi-cycle growth curves performed in A549 cells treated with a scrambled (scbl) or IFIH1- specific siRNAs. Cells were infected (MOI=0.01) with mIAV and supernatants collected and plaqued at the indicated time points. Error bars, SD, *p<0.05.

(B) Multi-cycle growth curves as described in (A) using wild type IAV in place of mIAV.

(C) Western Blot of whole cell extract derived from A549 cells treated as in (A) and subsequently transfected with IAV RNA and/or administered IFNβ. Immunoblots were probed for MDA5, IFIT1, and Actin.