Table 2.
List of WRN-Interacting Proteins Identified by Mass Spectrometry Using Benzonase and RNAse A in the Lysis Buffer
| number of unique peptides |
|||
|---|---|---|---|
| gene name | First IP | Second IP | description |
| WRN | 70 | 77 | Werner syndrome gene product |
| PRKDC | 62 | 53 | DNA-dependent protein kinase catalytic subunit |
| TMPO | 29 | 29 | Thymopoietin (lamina-associated polypeptide 2) |
| XRCC5 | 21 | 17 | KU86 ATP-dependent DNA helicase 2 subunit 2 |
| HNRNPU | 16 | 28 | Heterogeneous nuclear ribonucleoprotein U |
| XRCC6 | 15 | 18 | KU70 ATP-dependent DNA helicase 2 subunit 1 |
| DHX9 | 13 | 13 | DHX9 ATP-dependent RNA helicase A (DNA helicase II) |
| RPS3 | 9 | 14 | 40S ribosomal protein S3 |
| RALY | 9 | 7 | Autoantigenic hnRNP-associated with lethal yellow |
| RPA1 | 7 | 11 | Replication protein A 70 kDa DNA-binding subunit |
| RPS9 | 7 | 11 | 40S ribosomal protein S9 |
| LIG3 | 7 | 6 | DNA ligase 3 |
| DDX21 | 6 | 3 | Nucleolar RNA helicase 2 |
| HNRNPM | 6 | 5 | Heterogeneous nuclear ribonucleoprotein M |
| PARP-1 | 5 | 7 | Poly [ADP-ribose] polymerase 1 |
| RFC4 | 5 | 2 | Replication factor C subunit 4 |
| RPS3A | 5 | 4 | 40S ribosomal protein S3A (v-fos effector protein 1) |
| VRK3 | 4 | 7 | Vaccinia related kinase 3 |
| HIST1H1E | 4 | 7 | Histone 1, H1e |
| HIST1H4A | 4 | 6 | Histone H4 |
| DDX3X | 4 | 5 | DDX3X ATP-dependent RNA helicase |
| THOC1 | 4 | 2 | THO complex 1 (nuclear matrix protein p84) |
| RPL5 | 4 | 3 | 60S ribosomal protein L5 |
| TRIM28 | 3 | 4 | Tripartite motif-containing 28 |
| PELP1 | 3 | 4 | Proline, glutamate and leucine rich protein 1 |
| RBMX | 3 | 3 | HnRNP G, N-terminally processed |
| BAT1 | 3 | 2 | HLA-B associated transcript 1 |
| TOP1 | 3 | 2 | DNA topoisomerase 1 |
| RPA2 | 3 | 2 | Replication protein A 32 kDa subunit |
| CCT8 | 3 | 2 | T-complex protein 1 subunit theta |
| HNRNPUL2 | 2 | 4 | HnRNP U-like protein 2 |
| TFAM | 2 | 3 | Transcription factor A, mitochondrial |
| HIST1H2BK | 2 | 3 | Histone H2B type 1-K |
| RPL11 | 2 | 2 | 60S ribosomal protein L11 |
| SUPT16 | 2 | 2 | Facilitates chromatin remodeling subunit SPT16 |
| DDX1 | 2 | 2 | DDX1 ATP-dependent RNA helicase |
| HSPA5 | 2 | 2 | Heat shock 70 kDa protein 5 (glucose-regulated) |
| SSRP1 | 2 | 2 | Structure specific recognition protein 1 |
| HERC2 | 2 | 2 | Hect domain-containing protein 2 (E3 ubiquitin ligase) |
| SFRS1 | 2 | 2 | Splicing factor, arginine/serine-rich 1 |
| HIST1H2AB | 2 | 2 | Histone H2A type 1-B/E |