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ssDNA annealing activity of WRN C-terminal fragments. (A, B, and C) ssDNA annealing assays were performed as described in Figure 2, except protein was titrated in 2-fold serial dilutions from 0.5 to 16 nM. (D) ssDNA annealing activity was quantified by PhosphorImager analysis, and the percentage of dsDNA was calculated. Data values are the mean of at least two independent measurements.
