Table 2.
Sample Name |
Plant part | Sesamin Concentrationa | Asarinin Concentration | ||
---|---|---|---|---|---|
yieldb (ppt) ± SD |
% in extractc ± SD |
yield (ppt) ± SD |
% in extract ± SD |
||
Sample 1 | Root | 3.1 ± 0.3 | 8.8 ± 1.0 | 1.7 ± 0.1 | 4.7 ± 0.4 |
Sample 2 | Leaf/stem | 0.13 ± 0.02 | 0.95 ± 0.14 | 0.13 ± 0.02 | 0.9 ± 0.2 |
Sample 3 | Root | 1.7 ± 0.2 | 8.8 ± 0.9 | 1.1 ± 0.1 | 5.9 ± 0.2 |
Concentrations were determined by LC - MS analysis of extracts prepared from the relevant plant parts. Standard deviations are for triplicate analyses of the same extract. Extract concentration was calculated based on linear regression analysis of 6 point calibration curves of peak area versus concentration with slope (m) = 587881 ± 4628, intercept (b) = −20512 ± 10554, and R2 = 0.9998 for sesamin and a slope (m) = 647726 ± 16450, intercept (b) = −15594 ± 37516, and R2 = 0.9974 for asarinin. Extracts were diluted so that the concentrations tested fell within the linear range of the calibration curve.
Yield is reported as parts per thousand or mass of pure compound (g) per mass of original plant material (kg).
% in extract is reported as mass (g) of sesamin or asarinin per mass of solid extract (g) × 100.
The % in extract values are provided for the purpose of comparison with biological data.