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. Author manuscript; available in PMC: 2016 Apr 1.
Published in final edited form as: Lab Invest. 2015 Jul 6;95(10):1145–1156. doi: 10.1038/labinvest.2015.77

Figure 6. Galectin 3 inhibition results in the decrease in RhoA GTPase activity and actin reorganization in HCC cells.

Figure 6

Hepa1-6 cells were transfected with either scrambled or galectin 3 siRNA. RhoA GTPase activity was assessed by pull-down of the GTP- RhoA in non-transfected (NT), scrambled or galectin 3 siRNA-transfected cells. RhoA activation was significantly decreased after galectin 3 siRNA transfection (B, C, mean±SEM, N=4, *p<0.05). The RhoA activation was examined in wt and galectin 3−/− tumors as well (D, E). Less active RhoA was seen in the knockout tumors.

Immunofluorescence staining was performed to assess RhoA (A: b, f, j, green) localization and phalloidin (A: a, e, i, red) to visualize F-actin filaments. RhoA/F-actin was localized to the leading edges in the non-transfected (NT) and scrambled siRNA transfected cells (arrows). The galectin 3 siRNA-transfected cells had a more rounded shape with no lamellipodia formation and the RhoA staining was mainly cytoplasmic (Bar=20 μm).