Fig. 3.

Axonal injury at 2 days after blast. A–E) Horizontal sections immune stained for βAPP and using the RMO14 antibody. Rostral is toward the left. Panel A shows nearly the entire length of the lateral white matter on one side of a T6–9 strip of spinal cord. B–D are higher magnification views of the areas marked by boxes in A. βAPP+ axonal profiles are most numerous in the rostral third of the strip, whereas RMO14+ axonal profiles are found through the entire length of the strip. Note the large βAPP+ terminal bulb in panel C, and the linear arrays of RMO14+ profiles in panel D. There are no double-labeled axonal profiles. E shows the ventral white matter from ~T8–10 of a different mouse. The only βAPP+/RMO+ profile in E is marked by an arrow. Asterisks mark blood vessels at the ventral fissure. F) Transverse section from the same mouse as in A–D, at the T10 level, shows that RMO14+ profiles are extremely abundant in the lateral and ventral white matter. Optical density scale is shown inverted, so that RMO14+ profiles appear black. The level of background fluorescence has been increased so that the gray matter and section boundaries are visible, in order to provide orientation. Scale bar in F = 200 μm for A, E and F; 67 μm for B–D.