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. Author manuscript; available in PMC: 2015 Sep 29.
Published in final edited form as: Exp Neurol. 2015 May 7;271:53–71. doi: 10.1016/j.expneurol.2015.04.023

Fig. 6.

Fig. 6

Microtubule breakage and neurofilament compaction in the lateral and ventral white matter. A) Most βAPP+ axonal profiles appear swollen when viewed for TuJ1 immunofluorescence (arrows). A few additional, obviously swollen TuJ1+ axonal profiles are present but do not label for βAPP (arrowheads). B–E) The proportion of swollen TuJ1+ axonal profiles that are also RMO14+ (arrows) increases progressively with time after blast. Arrowheads indicate swollen TuJ1+ axons/axonal bulbs that do not label with the RMO14 antibody. Asterisks in B, C, and E mark several RMO14+ profiles that do not have a swollen appearance when viewed for TuJ1 immunolabeling. F) TuJ1 immunolabeling, visualized with Alexa 647, shows several axonal bulbs (arrows), two of which are situated in the midst of gaps in the tissue (asterisks). G) The same field of view as F, now showing the background fluorescence of the tissue in the green channel, captured using high laser power and gain and with the contrast further enhanced in Adobe Photoshop. The same axonal bulbs immunostained with TuJ1 in panel F are visible (arrows) and the empty spaces they occupy are obvious (asterisks). Small arrows mark blood vessels at the midline. The intense green labeling corresponds to a few YFP+ axons in the ventral component of the CST. Scale bar in G = 100 μm for all panels.