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. Author manuscript; available in PMC: 2015 Sep 29.
Published in final edited form as: Exp Neurol. 2015 May 7;271:53–71. doi: 10.1016/j.expneurol.2015.04.023

Fig. 7.

Fig. 7

Spectrin proteolysis by calpain, visualized by SBDP150 immunolabeling. A) At 6 h after blast, several YFP+ bulbs in the dCST are labeled for SBDP150 (arrows). Arrowhead marks a SBDP150+ bulb that does not show obvious accumulation of YFP. B) Swollen TuJ1+ axonal profiles in the lateral white matter show intense SBDP150 immunolabeling along their margins at 6 h post-blast (arrows). Note that the images in panels A and B are shown at higher magnifications than those in panels C–F; accordingly, the swollen axonal profiles indicated by arrows in panel B are similar in size to those marked by arrowheads in panels E–F. C, D) At 1 day after blast, several axonal profiles label very intensely for SBDP150 but appear otherwise normal (asterisks). TuJ1 immunolabeling in C confirms that they indeed correspond to axons. In D, RMO14 immunolabeling of the same field of view shows that the SBDP150+ axonal profiles are a separate population from those labeled with RMO14. A few very large and numerous smaller RMO14+ axonal profiles can also be seen. E, F) At 2 days after blast, some SBDP150+ axonal profiles appear swollen (arrowheads) whereas others appear vacuolated and also label with RMO14 (arrows). E shows single labeling because the thin rim of SBDP150 underlying the axolemma is largely obscured by the RMO14 immunolabeling in F. Also note for panels D and F that RMO14+ axonal profiles are more abundant than SBDP150+ axonal profiles. Scale bar in F = 67 μm for A and B, 100 μm for C–F.