Table 3. Detection of KRAS mutant clones in FFPE tissue DNA using next generation sequencing (NGS) and KRAS multiplex digital PCR assays.
ID | KRAS genotype | NGS mutant allele frequency (%) | Digital PCR multiplex mutant allele frequency (%) |
---|---|---|---|
S007 | G12C | 30.4 | 29.5 |
S027 | G12C | 48 | 53.8 |
F007 | G12D | 4 | 4.6 |
S002 | G12A | 32 | 30 |
S010 | G12C | ND | 0.3 |
G12A | 8.8 | 8.7 | |
S011 | G12V | 33.1 | 32.8 |
G12D | ND | 0.05 | |
S018 | G12F | 29 | 29.5 |
S030 | G13C | 17 | 18.6 |
S035 | G12C | 30 | 19.8 |
F124 | G12A | 23 | 23.7 |
F130 | G12V | 28 | 34.2 |
Key: ND- not detected.