Table 4. Detection of KRAS mutant clones in biclonal gDNA samples using next generation sequencing (NGS) and KRAS multiplex digital PCR assays.

Two individual library preparations were analysed for each sample.

ID	KRAS genotype	Library 1 NGS mutant allele frequency (%)	Library 2 NGS mutant allele frequency (%)	Digital PCR mutant allele frequency (%)
C1	G12C	25	23	19.4
	G12S	ND	ND	1.9
C2	G12C	26	26	18.4
	G12D	ND	ND	0.49
C3	G12D	9	10	9.8
	G12D	ND	ND	0.58

Key: ND- not detected.