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. 2015 Aug 3;95(10):1117–1129. doi: 10.1038/labinvest.2015.94

Figure 2.

Figure 2

The effects of t-PA on HSC activation are mediated by LRP1-dependent signaling. Whole-cell lysates either immunoprecipitated with anti-LRP1 followed by western blot for phospho-tyrosine and LRP1 in parallel (a), or directly analyzed by western blot with antibodies against p-LRP1 and total LRP1 (b). (c and d) HSC-T6 cells pretreated with PI3K pathway inhibitor LY294002 or DMSO for 60 min before co-treatment with t-PA or vehicle for 10 min (c) or 24 h (d). (e and f) HSC-T6 cells acid-washed (pH 5) at 4 °C to dissociate endogenous ligands, pretreated with the LRP1 inhibitor RAP or vehicle for 30 min, then co-treated with t-PA or vehicle for 10 min (e) or 24 h (f). (n=6 and 3 for d and f, respectively.) Panels compared in a, c, and e are noncontiguous lanes from single blots. Error bars±s.e.m. *P<0.05 and **P<0.01, Student's paired t-test.