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. Author manuscript; available in PMC: 2016 Sep 1.
Published in final edited form as: Exp Neurol. 2015 Jul 17;271:401–408. doi: 10.1016/j.expneurol.2015.07.010

Figure 1.

Figure 1

Progesterone protects endothelial cell population 3 days post-tMCAO. (A) Representative images of staining for endothelial cells using blood-brain barrier (SMI 71) from the cerebral cortex 3 days post-tMCAO and progesterone (P4)/vehicle (veh) treatment. Top panel shows endothelial cell (red) density in the contralateral (control) region of the ischemic brain while the lower panel shows endothelial cell density on the ipsilateral ischemic side of the cerebral cortex (box on Cresyl violet stained section shows region of interest for immunostaining). (B) Endothelial cell density normalized to contralateral hemisphere. tMCAO reduced endothelial cell density 3 days post-injury compared to sham controls, while progesterone significantly increased endothelial cell density over vehicle controls. (C) Representative images from the cerebral cortex of progesterone-treated animals analyzed for angiogenesis. VEGF (red) does not co-localize with endothelial cells (green). *p <0 .05, **p<0.001; n = sham (6), tMCAO + veh (7), P4 (8)