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. Author manuscript; available in PMC: 2016 Nov 1.
Published in final edited form as: Physiol Behav. 2015 Jul 17;151:155–161. doi: 10.1016/j.physbeh.2015.07.019

Table 3.

Product sequences for the elongated primers derived from the original forward and reverse. See “Materials and Methods: qPCR” for detailed explanation.

Direction Primer Sequence Product Product Sequence
NT
Original		 Forward ATGACCCTGCTAAATGTCTG 70 bp CAATGNNTANCANNNGAAGGGG
GAANNGGTAAAGGTAAAA
Reverse CTACCTCTACTGTTTCCCCC NNCCCGGTNNNGTGNTTGNNGGA
ATGGCCGGANCATTTTT
NT Elongated
Derived from
Original Forward		 Forward ATGACCCTGCTAAATGTCTG 196 bp ACATAACATCAGNATGGGGGAAA
CAGNTAGAGGTAGGATGAGGAGG
GATCTGGNTTCCAGGGAAGACAGT
TCCCTGCTGCTCTGGACGGCTTCA
GCTTGGAAGCAATGCTGACAATAT
ATCAACTCCAGAAAGTTTGCCACA
GCAGAGCCTTTCAGCATTGGGAGT
A
Reverse ACTCCCAATGCTGAAAGGC
NT Elongated
Derived from
Original Reverse		 Forward TGCTCAGATTCAGAAGAGGA 169 bp CGATTTATTGACCACTATGGTNAC
ATCAAAGATTAACAGAGCAAAACT
TCCTTACTGGAAAATGACCCTGCTA
AATGTCTGCAATCTTGTCAACAACA
TAAACAATCAGATGGGGGAAACA
GTAGAGGTAGA
Reverse CTACCTCTACTGTTTCCCCC