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. 2015 Sep 29;6:1062. doi: 10.3389/fmicb.2015.01062

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Copyright © 2015 Luedtke and Bosilevac.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Comparison of total EHEC estimated from select veal hide and carcass samples using qPCR, MPN, and dPCR assays targeting ecf1. (A) Total EHEC from selected hide samples were enumerated using the dPCR assay and compared to the enumeration values returned by the qPCR and MPN assays. (B) Total EHEC from selected carcass samples were enumerated using the dPCR assay and compared to the enumeration values returned by the qPCR and MPN assays. Error bars indicate the 95% confidence interval for each sample. A 95% confidence interval was not calculated for samples with a single Cq value from the qPCR assay.