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. 2015 Sep 29;6:777. doi: 10.3389/fpls.2015.00777

FIGURE 5.

FIGURE 5

Effect of CsA on HL-induced PsbO gene expression and its GTPase activation in Arabidopsis. (A) qRT-PCR analysis of PsbO1 and PsbO2 gene expression in CsA-pretreated detached leaves of WT plants after HL treatment. Total RNAs were extracted from the HL-treated detached leaves at the indicated time for the qRT-PCR analysis. (B) GTPase activity analysis using CsA-pretreated PSII membranes of WT, psbo1 and psbo2 after HL treatment. Detached Arabidopsis thaliana leaves or PSII membranes were pre-incubated with or without CsA (10 μM), and then treated with HL. WT GTPase activity at control light was set to 1. Different letters indicate statistically signifcant differences between treatments (Duncan’s multiple range test: P < 0.05). Asterisks () indicate a significant difference from the Control at P < 0.05, ∗∗P < 0.01.