FIGURE 7.

Effect of ROS and Ca²⁺-CaM3 cascade on CYP38 gene expression. (A) Sub-cellular localization and content of HL-induced Ca²⁺ production. Protoplasts were treated with or without HL for the indicated time period, then incubated with Fluo-3-AM for 30 min at room temperature, and observed using LCSM as described in materials and methods. Chloroplast autofluorescence is false colored red, and Fluo-3 fluorescence is false colored green. Scale bars = 10 μm. (B) Kinetics of changes in ROS and Ca²⁺ level by flow cytometry analysis after HL treatment. (C) Changes in CAM3 gene expression in HL-treated Arabidopsis WT plants. (D) CYP38 gene expression in the detached leaves of WT with or without AsA. (E) CYP38 gene expression in the detached leaves of WT with or without BAPTA-AM (1 mM) or CPA (1 μM) in response to HL treatment. Total RNAs were extracted from the HL-treated plants at the indicated time for the qRT-PCR analysis. Before HL treatment, the protoplasts and detached leaves were incubated with AsA at 1 mM final concentration. values for three replicates and asterisks (^∗) indicate a significant difference from the Control at ^∗P < 0.05, ^∗∗P < 0.01.